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Registro Completo |
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Biblioteca(s): |
Embrapa Amazônia Oriental. |
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Data corrente: |
30/11/2020 |
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Data da última atualização: |
07/12/2020 |
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Tipo da produção científica: |
Autoria/Organização/Edição de Livros |
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Autoria: |
CROUZEILLES, R.; RODRIGUES, R. R.; STRASSBURG, B. B. N.; BRANCALION, P. H. S.; GARCIA, L. C.; CHAVES, R. B.; CALMON, M.; CAMPOS, M. M. de; MACEDO, M.; LUI, G. H.; SCARAMUZZA, C. A. de M.; RIBEIRO, S.; PUGLIESE, L.; JUNQUEIRA, R. G. P.; PIÑA-RODRIGUES, F.; JAKOVAC, C.; MORAES, L. F. D. de; VIEIRA, D. L. M.; SAMPAIO, A. B.; GANADE, G.; OVERBECK, G. E.; ADAMS, C.; MELO, F.; FERREIRA, J. N.; MAIER, T.; OLIVEIRA, M.; SPOSITO, T. C.; METZKER, T.; TEDESCO, A. M.; BALDERI, A. P.; BALDERI, F.; PRUDENTE, C. M.; WIENS, I. K.; OLIVEIRA, A.; GUIMARÃES, T. M. G.; MARTINS, N. de M.; SANTIAMI, E.; MEDINA, A.; GARCIA, E.; BENINI, R.; SCHIMITT, J.; VIEIRA, R. M.; FERRARINI, O. G.; TAVARES, R. N. |
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Afiliação: |
Renato Crouzeilles, Instituto Internacional para Sustentabilidade / PUC-Rio / UFRJ; Ricardo Ribeiro Rodrigues, LERF/LCB, Escola superior de Agricultura Luiz de Queiroz/ESALQ / USP; Bernardo B. N. Strassburg, Instituto Internacional para Sustentabilidade / PUC-Rio / UFRJ; Pedro Henrique Santin Brancalion, ESALQ / USP; Letícia Couto Garcia, UFMS; Rafael Barreiro Chaves, Secretaria de Infraestrutura e Meio Ambiente/São Paulo; Miguel Calmon, World Resource Institute – Brasil; Marina Merlo Sampaio de Campos, The Nature Conservancy; Marcio Macedo, BNDES; Gabriel Henrique Lui, Instituto Chico Mendes de Conservação da Biodiversidade/ Ministério do Meio Ambiente; Carlos Alberto de Mattos Scaramuzza, Flexus Consultoria em Biodiversidade e Sustentabilidade; Severino Ribeiro, Centro de Pesquisas Ambientais do Nordeste / Pacto pela Restauração da Mata Atlântica; Ludmila Pugliese, ESALQ/USP / Pacto pela Restauração da Mata Atlântica; Rodrigo Gravina P. Junqueira, Instituto Socioambiental; Fatima Piña-Rodrigues, UFSCAR; Ana Catarina Jakovac, Instituto Internacional para Sustentabilidade Wageningen University; LUIZ FERNANDO DUARTE DE MORAES, CNPAB; DANIEL LUIS MASCIA VIEIRA, Cenargen; Alexandre Bonesso Sampaio, Instituto Chico Mendes de Conservação da Biodiversidade/ Ministério do Meio Ambiente; Gislene Ganade, UFRN; Gerhard E. Overbeck, UFRS; Cristina Adams, USP; Felipe Melo, UFPE; JOICE NUNES FERREIRA, CPATU; Thais Maier, The Nature Conservancy; Mariana Oliveira, World Resource Institute – Brasil; Tereza Cristina Sposito, Instituto Bem Ambiental; Thiago Metzker, Instituto Bem Ambiental; Anazélia M. Tedesco, The University of Queensland; Ana Paula Balderi, Instituto Copaíba; Flavia Balderi, Instituto Copaíba; Claudia Mascagni Prudente, Instituto Auá; Ivy K. Wiens, Instituto Socioambiental; Andrea Oliveira, Instituto Çarakura; Tatiana Motta Grilo Guimarães, Projetos Corredores Vale do Paraíba; Narliane de M. Martins, Instituto Federal do Paraná; Edson Santiami, ESALQ/USP; Aretha Medina, Fundação SOS Mata Atlântica; Edenise Garcia, The Nature Conservancy; Rubens Benini, The Nature Conservancy; Jair Schimitt, Departamento de Florestas e Combate ao Desmatamento/ Secretaria de Mudança do Clima e Floresta; Rodrigo M. Vieira, Ministério do Meio Ambiente; Otavio G. Ferrarini, Ministério do Meio Ambiente; Roberto N. Tavares. |
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Título: |
Relatório temático sobre restauração de paisagens e ecossistemas. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
São Carlos, SP: Editora Cubo, 2019. |
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Páginas: |
76 p. |
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ISBN: |
978-85-60064-91-5 |
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Idioma: |
Português |
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Palavras-Chave: |
Paisagem; Restauração. |
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Thesagro: |
Biodiversidade; Ecossistema; Meio Ambiente; Política Ambiental. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/218369/1/Relatorio-Restauracao-VF.pdf
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Marc: |
LEADER 01869nam a2200709 a 4500
001 2127206
005 2020-12-07
008 2019 bl uuuu t 00u1 u #d
020 $a978-85-60064-91-5
100 1 $aCROUZEILLES, R.
245 $aRelatório temático sobre restauração de paisagens e ecossistemas.$h[electronic resource]
260 $aSão Carlos, SP: Editora Cubo$c2019
300 $a76 p.
650 $aBiodiversidade
650 $aEcossistema
650 $aMeio Ambiente
650 $aPolítica Ambiental
653 $aPaisagem
653 $aRestauração
700 1 $aRODRIGUES, R. R.
700 1 $aSTRASSBURG, B. B. N.
700 1 $aBRANCALION, P. H. S.
700 1 $aGARCIA, L. C.
700 1 $aCHAVES, R. B.
700 1 $aCALMON, M.
700 1 $aCAMPOS, M. M. de
700 1 $aMACEDO, M.
700 1 $aLUI, G. H.
700 1 $aSCARAMUZZA, C. A. de M.
700 1 $aRIBEIRO, S.
700 1 $aPUGLIESE, L.
700 1 $aJUNQUEIRA, R. G. P.
700 1 $aPIÑA-RODRIGUES, F.
700 1 $aJAKOVAC, C.
700 1 $aMORAES, L. F. D. de
700 1 $aVIEIRA, D. L. M.
700 1 $aSAMPAIO, A. B.
700 1 $aGANADE, G.
700 1 $aOVERBECK, G. E.
700 1 $aADAMS, C.
700 1 $aMELO, F.
700 1 $aFERREIRA, J. N.
700 1 $aMAIER, T.
700 1 $aOLIVEIRA, M.
700 1 $aSPOSITO, T. C.
700 1 $aMETZKER, T.
700 1 $aTEDESCO, A. M.
700 1 $aBALDERI, A. P.
700 1 $aBALDERI, F.
700 1 $aPRUDENTE, C. M.
700 1 $aWIENS, I. K.
700 1 $aOLIVEIRA, A.
700 1 $aGUIMARÃES, T. M. G.
700 1 $aMARTINS, N. de M.
700 1 $aSANTIAMI, E.
700 1 $aMEDINA, A.
700 1 $aGARCIA, E.
700 1 $aBENINI, R.
700 1 $aSCHIMITT, J.
700 1 $aVIEIRA, R. M.
700 1 $aFERRARINI, O. G.
700 1 $aTAVARES, R. N.
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Registro original: |
Embrapa Amazônia Oriental (CPATU) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/01/2020 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
OLIVEIRA, C. S.; BARROS, B. A. F. de; MONTEIRO, C. A. S.; ROSA, P. M. S.; LEAL, G. R.; SERAPIÃO, R. V.; CAMARGO, L. S. de A. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; B. A. F. de Barros; C. A. S. Monteiro; P. M. S. Rosa; G. R. Leal; Raquel Varela Serapião; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Individual assessment of bovine embryo development using a homemade chamber reveals kinetic patterns of success and failure to reach blastocyst stage. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Systems Biology in Reproductive Medicine, v. 65, n. 4, p. 301-311, 2019. |
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DOI: |
https://doi.org/10.1080/19396368.2019.1589601 |
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Idioma: |
Inglês |
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Conteúdo: |
Most early developmental data are lost in bovine embryo culture systems. We developed and validated a method for culture of bovine embryos in groups that allow individual assessment. An autoclavable low-cost multiembryo chamber (MEC) was prepared using a polyester mesh fixed to a glass coverslip. Embryonic development was not affected by MEC. Compared to conventional bovine culture system (oil-covered drops, control), cleavage (C, 71.2 ± 7.8%; MEC, 74.3 ± 6.0%), blastocyst rate (C, 29.9 ± 4.4%; MEC, 28.3 ± 5.0%) and blastocyst cell number (C, 94.1 ± 9.7; MEC, 92.9 ± 5.3) were similar. Caspase 3 positive cell index in blastocysts was increased in MEC group, but apoptosis rate was below 5% (C, 2.9 ± 0.5; MEC, 4.6 ± 0.6). Using MEC, we performed a retrospective analysis for 'failure' and 'success' embryos, based on their ability to reach the blastocyst stage. We detected the majority of 'success' embryos displayed 8 cells at 48 h post-insemination (hpi) (48.7%), but blastocysts derived from this pattern presented lower cell numbers (91.3 ± 4.2 vs. 107.9 ± 4.9) and higher apoptosis index (6.2 ± 0.6 vs. 4.4 ± 0.5) than blastocysts from 4-cell embryos at 48 hpi. Most (72.0%) embryos that were at morula stage 120 hpi reached blastocyst stage at 168 hpi. Those blastocysts presented more number of cells than blastocysts derived from embryos exhibiting 16 cells at 120 hpi (108.6 ± 4.1 vs. 83.9 ± 4.8). Combination of embryo kinetics data at 48 and 120 hpi revealed high chances of blastocyst formation for patterns: 8 cells/morula, 4 cells/morula, 8 cells/16 cells and 4 cells/16 cells. Blastocysts formed from 4-cell/morula and 8-cell/morula patterns represented 69% of all 168 hpi blastocysts. Blastocysts derived from 4 cells/16 cells displayed decreased apoptosis (3.1 ± 0.6). Our results suggest that MEC can be used for bovine embryo culture without detrimental effects on development and can help to predict blastocyst formation and quality of in vitro fertilization (IVF) embryos. Abbreviations: BSA: bovine serum albumine; COC: cumulus-oocyte complex; FERT-TALP: Tyrode's albumin lactate pyruvate fertilization; FBS: fetal bovine serum; IVF: in vitro fertilization; MEC: multiembryo chamber; PBS: phosphate buffered saline; SOF-AA: synthetic oviductal fluid with amino acids medium; TCM: Tissue Culture Medium. MenosMost early developmental data are lost in bovine embryo culture systems. We developed and validated a method for culture of bovine embryos in groups that allow individual assessment. An autoclavable low-cost multiembryo chamber (MEC) was prepared using a polyester mesh fixed to a glass coverslip. Embryonic development was not affected by MEC. Compared to conventional bovine culture system (oil-covered drops, control), cleavage (C, 71.2 ± 7.8%; MEC, 74.3 ± 6.0%), blastocyst rate (C, 29.9 ± 4.4%; MEC, 28.3 ± 5.0%) and blastocyst cell number (C, 94.1 ± 9.7; MEC, 92.9 ± 5.3) were similar. Caspase 3 positive cell index in blastocysts was increased in MEC group, but apoptosis rate was below 5% (C, 2.9 ± 0.5; MEC, 4.6 ± 0.6). Using MEC, we performed a retrospective analysis for 'failure' and 'success' embryos, based on their ability to reach the blastocyst stage. We detected the majority of 'success' embryos displayed 8 cells at 48 h post-insemination (hpi) (48.7%), but blastocysts derived from this pattern presented lower cell numbers (91.3 ± 4.2 vs. 107.9 ± 4.9) and higher apoptosis index (6.2 ± 0.6 vs. 4.4 ± 0.5) than blastocysts from 4-cell embryos at 48 hpi. Most (72.0%) embryos that were at morula stage 120 hpi reached blastocyst stage at 168 hpi. Those blastocysts presented more number of cells than blastocysts derived from embryos exhibiting 16 cells at 120 hpi (108.6 ± 4.1 vs. 83.9 ± 4.8). Combination of embryo kinetics data at 48 and 120 hpi revealed high chances of blast... Mostrar Tudo |
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Palavras-Chave: |
Culture system; Embryo kinetics; Embryonic quality; Single embryo culture. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03220naa a2200253 a 4500
001 2118728
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1080/19396368.2019.1589601$2DOI
100 1 $aOLIVEIRA, C. S.
245 $aIndividual assessment of bovine embryo development using a homemade chamber reveals kinetic patterns of success and failure to reach blastocyst stage.$h[electronic resource]
260 $c2019
520 $aMost early developmental data are lost in bovine embryo culture systems. We developed and validated a method for culture of bovine embryos in groups that allow individual assessment. An autoclavable low-cost multiembryo chamber (MEC) was prepared using a polyester mesh fixed to a glass coverslip. Embryonic development was not affected by MEC. Compared to conventional bovine culture system (oil-covered drops, control), cleavage (C, 71.2 ± 7.8%; MEC, 74.3 ± 6.0%), blastocyst rate (C, 29.9 ± 4.4%; MEC, 28.3 ± 5.0%) and blastocyst cell number (C, 94.1 ± 9.7; MEC, 92.9 ± 5.3) were similar. Caspase 3 positive cell index in blastocysts was increased in MEC group, but apoptosis rate was below 5% (C, 2.9 ± 0.5; MEC, 4.6 ± 0.6). Using MEC, we performed a retrospective analysis for 'failure' and 'success' embryos, based on their ability to reach the blastocyst stage. We detected the majority of 'success' embryos displayed 8 cells at 48 h post-insemination (hpi) (48.7%), but blastocysts derived from this pattern presented lower cell numbers (91.3 ± 4.2 vs. 107.9 ± 4.9) and higher apoptosis index (6.2 ± 0.6 vs. 4.4 ± 0.5) than blastocysts from 4-cell embryos at 48 hpi. Most (72.0%) embryos that were at morula stage 120 hpi reached blastocyst stage at 168 hpi. Those blastocysts presented more number of cells than blastocysts derived from embryos exhibiting 16 cells at 120 hpi (108.6 ± 4.1 vs. 83.9 ± 4.8). Combination of embryo kinetics data at 48 and 120 hpi revealed high chances of blastocyst formation for patterns: 8 cells/morula, 4 cells/morula, 8 cells/16 cells and 4 cells/16 cells. Blastocysts formed from 4-cell/morula and 8-cell/morula patterns represented 69% of all 168 hpi blastocysts. Blastocysts derived from 4 cells/16 cells displayed decreased apoptosis (3.1 ± 0.6). Our results suggest that MEC can be used for bovine embryo culture without detrimental effects on development and can help to predict blastocyst formation and quality of in vitro fertilization (IVF) embryos. Abbreviations: BSA: bovine serum albumine; COC: cumulus-oocyte complex; FERT-TALP: Tyrode's albumin lactate pyruvate fertilization; FBS: fetal bovine serum; IVF: in vitro fertilization; MEC: multiembryo chamber; PBS: phosphate buffered saline; SOF-AA: synthetic oviductal fluid with amino acids medium; TCM: Tissue Culture Medium.
653 $aCulture system
653 $aEmbryo kinetics
653 $aEmbryonic quality
653 $aSingle embryo culture
700 1 $aBARROS, B. A. F. de
700 1 $aMONTEIRO, C. A. S.
700 1 $aROSA, P. M. S.
700 1 $aLEAL, G. R.
700 1 $aSERAPIÃO, R. V.
700 1 $aCAMARGO, L. S. de A.
773 $tSystems Biology in Reproductive Medicine$gv. 65, n. 4, p. 301-311, 2019.
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